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maxi lambda dna purification kit  (Qiagen)


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    Structured Review

    Qiagen maxi lambda dna purification kit
    Maxi Lambda Dna Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+dna+purification+kit/maxi+lambda+dna+purification+kit/pmc05772233-270-31-36
    Average 90 stars, based on 1 article reviews
    maxi lambda dna purification kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Molecular cloning and expression analysis of two distinct beta-glucosidase genes, bg1 and aven1, with very different biological roles from the thermophilic, saprophytic fungus Talaromyces emersonii.
    Article Snippet: Recent sequencing of a number of fungal genomes has revealed the presence of multiple putative b-glucosidases.. Here, we report the cloning of two b-glucosidase genes (bg1 and aven1), which have very different biological functions and represent two of a number of b-glucosidases from Talaromyces emersonii.. The bg1 gene, encoding a putative intracellular b-glucosidase, shows significant similarity to other fungal glucosidases from glycosyl hydrolase family 1, known to be involved in cellulose degradation.

    Article Title: Analysis of the Shotgun Expression Library of the Mycobacterium tuberculosis Genome for Immunodominant Polypeptides: Potential Use in Serodiagnosis
    Article Snippet: .. After subsequent clonal propagation and PFU determination, recombinant phage DNA was isolated and purified from selected positive clones by using the Qiagen lambda DNA purification kit according to the manufacturer's instructions. ..

    Purification:

    Article Title: Molecular cloning and expression analysis of two distinct beta-glucosidase genes, bg1 and aven1, with very different biological roles from the thermophilic, saprophytic fungus Talaromyces emersonii.
    Article Snippet: Recent sequencing of a number of fungal genomes has revealed the presence of multiple putative b-glucosidases.. Here, we report the cloning of two b-glucosidase genes (bg1 and aven1), which have very different biological functions and represent two of a number of b-glucosidases from Talaromyces emersonii.. The bg1 gene, encoding a putative intracellular b-glucosidase, shows significant similarity to other fungal glucosidases from glycosyl hydrolase family 1, known to be involved in cellulose degradation.

    Article Title: Isolation, characterization and complete nucleotide sequence of a novel temperate bacteriophage Min1, isolated from the nematode pathogen Microbacterium nematophilum.
    Article Snippet: Southern hybridization was performed using the NEBlot Phototope kit and the Phototope Star Detection kit (New England BioLabs, UK). .. Bacteriophage DNA was prepared using a lambda DNA purification kit (Qiagen), with overnight incubation in ammonium acetate (final concentration 2.5 M) at 4 C. Purified DNA was digested with BamHI and ligated into vector pUC18 previously linearized by the same enzyme. ..

    Article Title: Tissue inhibitor of metalloproteinase type three (TIMP-3) composition and methods
    Article Snippet: .. DNA from bacteriophage lambda was purified using a Qiagen Lambda DNA purification kit (Chatsworth, Calif.), Plate lystates from 10 confluent 135 mm petri dishes were pooled for each specimen. ..

    Article Title: Tissue inhibitor of metalloproteinase type three (TIMP-3) composition and methods
    Article Snippet: .. DNA from bacteriophage lambda was purified using a Qiagen Lambda DNA purification kit (Chatsworth, Calif.). ..

    Article Title: Genomic analysis of bacteriophage ε 34 of Salmonella enterica serovar Anatum (15+)
    Article Snippet: .. The mutant and wildtype DNAs were prepared and purified as described [ , ] or by using QIAGEN Lambda DNA Purification Kit (QIAGEN, Valencia CA). .. The DNA was sequenced commercially by Fidelity System Inc. (Gaithersburg MD) with the final sequence opened immediately upstream of the small terminase subunit gene to be in conformity with the presentation of other similar phages.

    Article Title: Tissue inhibitor of metalloproteinase type three (TIMP-3) composition and methods
    Article Snippet: .. DNA from bacteriophage lambda was purified using a Qiagen Lambda DNA purification kit (Chatsworth, Calif.), Plate lysates from 10 confluent 135 mm petri dishes were pooled for each specimen. ..

    Article Title: Analysis of the Shotgun Expression Library of the Mycobacterium tuberculosis Genome for Immunodominant Polypeptides: Potential Use in Serodiagnosis
    Article Snippet: .. After subsequent clonal propagation and PFU determination, recombinant phage DNA was isolated and purified from selected positive clones by using the Qiagen lambda DNA purification kit according to the manufacturer's instructions. ..

    Lambda DNA Preparation:

    Article Title: Molecular cloning and expression analysis of two distinct beta-glucosidase genes, bg1 and aven1, with very different biological roles from the thermophilic, saprophytic fungus Talaromyces emersonii.
    Article Snippet: Recent sequencing of a number of fungal genomes has revealed the presence of multiple putative b-glucosidases.. Here, we report the cloning of two b-glucosidase genes (bg1 and aven1), which have very different biological functions and represent two of a number of b-glucosidases from Talaromyces emersonii.. The bg1 gene, encoding a putative intracellular b-glucosidase, shows significant similarity to other fungal glucosidases from glycosyl hydrolase family 1, known to be involved in cellulose degradation.

    Article Title: Isolation, characterization and complete nucleotide sequence of a novel temperate bacteriophage Min1, isolated from the nematode pathogen Microbacterium nematophilum.
    Article Snippet: Southern hybridization was performed using the NEBlot Phototope kit and the Phototope Star Detection kit (New England BioLabs, UK). .. Bacteriophage DNA was prepared using a lambda DNA purification kit (Qiagen), with overnight incubation in ammonium acetate (final concentration 2.5 M) at 4 C. Purified DNA was digested with BamHI and ligated into vector pUC18 previously linearized by the same enzyme. ..

    Article Title: Tissue inhibitor of metalloproteinase type three (TIMP-3) composition and methods
    Article Snippet: .. DNA from bacteriophage lambda was purified using a Qiagen Lambda DNA purification kit (Chatsworth, Calif.), Plate lystates from 10 confluent 135 mm petri dishes were pooled for each specimen. ..

    Article Title: Tissue inhibitor of metalloproteinase type three (TIMP-3) composition and methods
    Article Snippet: .. DNA from bacteriophage lambda was purified using a Qiagen Lambda DNA purification kit (Chatsworth, Calif.). ..

    Article Title: Genomic analysis of bacteriophage ε 34 of Salmonella enterica serovar Anatum (15+)
    Article Snippet: .. The mutant and wildtype DNAs were prepared and purified as described [ , ] or by using QIAGEN Lambda DNA Purification Kit (QIAGEN, Valencia CA). .. The DNA was sequenced commercially by Fidelity System Inc. (Gaithersburg MD) with the final sequence opened immediately upstream of the small terminase subunit gene to be in conformity with the presentation of other similar phages.

    Article Title: Tissue inhibitor of metalloproteinase type three (TIMP-3) composition and methods
    Article Snippet: .. DNA from bacteriophage lambda was purified using a Qiagen Lambda DNA purification kit (Chatsworth, Calif.), Plate lysates from 10 confluent 135 mm petri dishes were pooled for each specimen. ..

    Article Title: Analysis of the Shotgun Expression Library of the Mycobacterium tuberculosis Genome for Immunodominant Polypeptides: Potential Use in Serodiagnosis
    Article Snippet: .. After subsequent clonal propagation and PFU determination, recombinant phage DNA was isolated and purified from selected positive clones by using the Qiagen lambda DNA purification kit according to the manufacturer's instructions. ..

    other:

    Article Title: Cloning of a pore-forming subunit of ATP-sensitive potassium channel from Clonorchis sinensis
    Article Snippet: Positive clones were isolated and the lambda DNA was purified by use of a Qiagen Lambda kit (Qiagen, Valencia, CA).

    Incubation:

    Article Title: Isolation, characterization and complete nucleotide sequence of a novel temperate bacteriophage Min1, isolated from the nematode pathogen Microbacterium nematophilum.
    Article Snippet: Southern hybridization was performed using the NEBlot Phototope kit and the Phototope Star Detection kit (New England BioLabs, UK). .. Bacteriophage DNA was prepared using a lambda DNA purification kit (Qiagen), with overnight incubation in ammonium acetate (final concentration 2.5 M) at 4 C. Purified DNA was digested with BamHI and ligated into vector pUC18 previously linearized by the same enzyme. ..

    Concentration Assay:

    Article Title: Isolation, characterization and complete nucleotide sequence of a novel temperate bacteriophage Min1, isolated from the nematode pathogen Microbacterium nematophilum.
    Article Snippet: Southern hybridization was performed using the NEBlot Phototope kit and the Phototope Star Detection kit (New England BioLabs, UK). .. Bacteriophage DNA was prepared using a lambda DNA purification kit (Qiagen), with overnight incubation in ammonium acetate (final concentration 2.5 M) at 4 C. Purified DNA was digested with BamHI and ligated into vector pUC18 previously linearized by the same enzyme. ..

    Plasmid Preparation:

    Article Title: Isolation, characterization and complete nucleotide sequence of a novel temperate bacteriophage Min1, isolated from the nematode pathogen Microbacterium nematophilum.
    Article Snippet: Southern hybridization was performed using the NEBlot Phototope kit and the Phototope Star Detection kit (New England BioLabs, UK). .. Bacteriophage DNA was prepared using a lambda DNA purification kit (Qiagen), with overnight incubation in ammonium acetate (final concentration 2.5 M) at 4 C. Purified DNA was digested with BamHI and ligated into vector pUC18 previously linearized by the same enzyme. ..

    Mutagenesis:

    Article Title: Genomic analysis of bacteriophage ε 34 of Salmonella enterica serovar Anatum (15+)
    Article Snippet: .. The mutant and wildtype DNAs were prepared and purified as described [ , ] or by using QIAGEN Lambda DNA Purification Kit (QIAGEN, Valencia CA). .. The DNA was sequenced commercially by Fidelity System Inc. (Gaithersburg MD) with the final sequence opened immediately upstream of the small terminase subunit gene to be in conformity with the presentation of other similar phages.

    Recombinant:

    Article Title: Analysis of the Shotgun Expression Library of the Mycobacterium tuberculosis Genome for Immunodominant Polypeptides: Potential Use in Serodiagnosis
    Article Snippet: .. After subsequent clonal propagation and PFU determination, recombinant phage DNA was isolated and purified from selected positive clones by using the Qiagen lambda DNA purification kit according to the manufacturer's instructions. ..

    Isolation:

    Article Title: Analysis of the Shotgun Expression Library of the Mycobacterium tuberculosis Genome for Immunodominant Polypeptides: Potential Use in Serodiagnosis
    Article Snippet: .. After subsequent clonal propagation and PFU determination, recombinant phage DNA was isolated and purified from selected positive clones by using the Qiagen lambda DNA purification kit according to the manufacturer's instructions. ..



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